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Protocols related to CRISPRi/a cell lines and library generation

CRISPRi/a cell line generation primer

Adapter sequences for appending to pooled sgRNA library oligo sequences

CRISPRi/a library cloning

Large scale lentivirus production protocol

Protocols related to CRISPRi/a screens and screen processing

Screening guidelines and example infection protocol, suspension cells

Screening guidelines and example infection protocol, adherent cells

Example and Template for cell measurements during growth screens, suspension and adherent cells

Preparation of Illumina sequencing sample after a CRISPR screen

ScreenProcessing pipeline for analysis of pooled CRISPR screens

Protocols related to generating individual CRISPRi/a sgRNA vectors

Template for ordering oligos for individual cloning

sgRNA cloning by annealing protocol